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KMID : 1094720220270050810
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Biotechnology and Bioprocess Engineering
2022 Volume.27 No. 5 p.810 ~ p.817
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Screening a Panel of Acid-producing Strains by Developing a High-throughput Method
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Lijuan Zhu
Hui Zhang
Shiyuan Wang
Anqi Zhao
Lingbo Qu
Wenlong Xiong
Md. Asraful Alam
Wenlong Ma
Yongkun Lv
Jingliang Xu
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Abstract
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Organic acids are natural cellular metabolites, which are widely used in food, pharmaceutical, and chemical industries. Among them, L-lactic acid is of special interest, because it is widely used in food and pharmaceutical industries and its monopolymer (poly (lactic acid)) is a green, renewable, biodegradable, and biocompatible alternative to the petroleum-based polymers. Currently, organic acids are predominantly produced by microbial fermentation. Their productions have been substantially improved by genetic modifications, metabolic engineering, and fermentation optimizations. However, the commonly used microbial producers still suffer from low acidic tolerance. Screening higher tolerant acid-producing microorganisms from the nature is relatively less explored. The traditional fermented foods are good resources for the screening of acid-producing and probiotic microorganisms. However, they are relatively less explored, especially those foods in developing countries. To speed up the acid-producing microorganism screening, we developed and validated a high-throughput method in this study. By using this method, we screened 1,296 colonies in 4 days and obtained a panel of acid-producing microorganisms. Among them, a Lacticaseibacillus rhamnosus showed the potential for organic acid production and probiotics applications.
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KEYWORD
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high-throughput screening, L-lactic acid, Lacticaseibacillus rhamnosus, acid-producing microorganisms, traditional fermentation foods
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